Description
Anti Cardiolipin IgG ELISA Assay Kit is based on the binding of antibodies on human serum or plasma directed against the Antigensic complex between Cardiolipina and β2-Glycoprotein; this complex is coated on the microplate. In the first step the antibodies present in calibrators, controls or prediluted samples bind to this coated Antigens. After a 60 minute incubation the microplate is washed with a wash buffer to remove the non-reactive serum or plasma components. Then an anti-human IgM horseradish peroxidase conjugated solution recognizes IgM class antibodies bound to the immobilized Antigenss. After a 60 minute incubation any excess of enzyme conjugate, which is not specifically bound, is washed away with the wash buffer. Finally, achromogenic substrate solution containing TMB is dispensed into the wells. After a 15 minute incubation the color development is stopped by adding the stop solution. The solution turns yellow at this point. The level of color is directly proportional to the concentration of IgG antibodies present in the original sample. The concentration of IgG antibodies in the sample is calculated through a calibration curve